Paph. Quantum Light—alba x Saint Swithin (half album)

[Ernesto]

Another attempt at alba multifloral hybrids. This time it’s an alba Brachypetalum hybrid as the pod parent

pollinated with my Saint Swithin with philippinense alba as a parent.


Pollination happened 10/13/22, pod was harvested May 2023 and sown 6/1/23.



I saw germination mid-July, then I split the protocorms into three groups for colchicine treatment on 8/5/23.



Just shy of 4 months after flasking and 2 months after colchicine treatment, here’s how the control group is doing



Here’s how the weak dose (2 uM colchicine, 4 weeks in the dark) is doing. I never got around the chance to removing the solution from their flask, but that doesn’t seem to have slowed them down too much



And lastly, the strong (50 uM colchicine for 5 days) group. Low survival, but the protocorms are green and (slowly) putting on size— no roots or leaves yet though. At the very least I know the colchicine dose did… something lol





That’s all for now!

 

[NYEric]

Nice.Good luck. Remember me at compot stage.

 

[Ozpaph]

Thanks for documenting your experiment.

 

[Ernesto]

Unfortunately the weak dose group ended up browning and died (keeping them submerged for several months probably did a number on em). However, the strong dose group is still around. I’m seeing some proliferation now with one patch growing leaves and another patch being entirely callus tissue at the moment.

 

[Ernesto]

I also deflasked the control group two months ago. Four compots!

 

[Happypaphy7]

Looking good!

 

[NYEric]

Good luck. Keep us posted.

 

[Ricardo Boricua]

Fascinating!