Tissue culture vessels

Discussion in 'Orchid Conservation' started by Slipper lover, Jun 8, 2017.

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  1. Jun 8, 2017 #1

    Slipper lover

    Slipper lover

    Slipper lover

    Well-Known Member

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    I am wondering what a good company and name of vessel you use and if It has good results
     
  2. Jun 8, 2017 #2

    Slipper lover

    Slipper lover

    Slipper lover

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    Thank you in advanced for the info and your responses
     
  3. Jun 23, 2017 #3

    TyroneGenade

    TyroneGenade

    TyroneGenade

    mad scientist

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    Hello,

    You can use these:
    [​IMG]
    Surface sterilize them with 3% hydrogen peroxide and then leave them in sunlight to break down the peroxide. I would make up 100 mL of 3% hydrogen peroxide with a drop of tween-20 (or dishwashing soap). Use spray bottle to spray all the surfaces. Do this in your laminar flow hood (or whatever sterile space you are using for sowing seed). Put the lids on the tubs and then place these in a large plastic bag. Spray the inside of the bag thoroughly to make sure everything is sterile and then seal and place these in sunlight. The thin polypropylene naturally breathes so there is no need to vent them.

    I used this method myself and had excellent results. I had tubs remain sterile for years (and were thrown out in the trash sterile when I finally gave up on the seed or age dried out).

    See http://www.orchidforum.eu/viewtopic.php?f=26&t=1292&start=40#p14808 for more DIY home orchid seed sowing.

    Hope this helps.
     
  4. Jun 23, 2017 #4

    PaphMadMan

    PaphMadMan

    PaphMadMan

    phytomanic

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    Tyrone's suggestion is good, and inexpensive, but if you're more comfortable purchasing sterile containers meant for the purpose https://phytotechlab.com/ is a reliable company with many good choices.
     

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