Paphiopedilum Saint Swithin (rothschildianum x philippinense frma. album) x delenatii frma. alba

Slippertalk Orchid Forum

Help Support Slippertalk Orchid Forum:

Ernesto

ST Supporter
Supporting Member
Joined
Apr 21, 2020
Messages
158
Reaction score
79
Location
Manhattan, NY
I want to make a Paph hybrid that looks like a white rothschildianum. To get there, I figured I needed a way to get a roth shape, a way to wipe out anthocyanin, a way to wipe out carotenoids, and a way to wipe out chlorophyll. I think there are many ways to do this, and this is the first route I’ve taken that has yielded promising results!
60021F72-A79F-4EC5-B44A-DC356F2A7075.jpeg
This is the furthest along I’ve gotten with Paphiopedilum breeding, so I wanted to share what I’ve got so far. I’ve germinated seeds via green pod method of Paph. Saint Swithin (roth x philippinense alba) x delenatii alba. Based on what I’ve seen online, the philippinense albino allele works in tandem with the delenatii albino allele to produce white to near white flowers in the offspring— philippinense alba lacks pigment save for chlorophyll, while delenatii suppresses yellows and greens (and the albinos lack anthocyanin!). Together, they make (poorly shaped) white flowers. The Saint Swithin I used as the pod parent should contribute an albino allele, as well as a “roth-y” shape and substance. It isn’t an award winning flower though, so to compensate for its shape I used a very nice delenatii alba as the pollen parent.
2BEA985D-C508-4C9A-8C3A-9264D2975E3B.jpeg
5F8952F3-CA52-484A-83BC-F026B2134F5F.jpeg

The protocorms are now beginning to photosynthesize and I’m making preparations for replates soon!

47C7A1FE-10AA-4884-BE10-512E1A3C4C43.jpeg
 

Ozpaph

Well-Known Member
Joined
Jan 15, 2011
Messages
18,705
Reaction score
1,528
Location
Brisbane, Australia
good luck. Sounds like a long breeding timeline.
Why not use the 'half' albino SS sibs to start. At least the shape will be 'roth like'.
AFAIK there are no pure white multiflorals so white might be impossible without a spontaneous mutation.
 

TyroneGenade

mad scientist
Joined
Sep 15, 2008
Messages
2,421
Reaction score
112
Location
Johnson City, TN
What medium did you use?

When you replace make sure no ethanol touches the the plants or gets into the replates. It is better to use hydrogen peroxide as service sterilant while working with the flasks if you have limited space.
 

Ernesto

ST Supporter
Supporting Member
Joined
Apr 21, 2020
Messages
158
Reaction score
79
Location
Manhattan, NY
good luck. Sounds like a long breeding timeline.
Why not use the 'half' albino SS sibs to start. At least the shape will be 'roth like'.
AFAIK there are no pure white multiflorals so white might be impossible without a spontaneous mutation.
Thanks, perhaps I’ll see flowers before I’m 40 😅

It’s an option for the next time the plant is in bloom— since it was a young plant I decided to only have it hold one pod, and I went with one that would yield a higher amount of white to white-ish flowers. The half album plants are both Aa, so assuming the albino mutation is at the same locus in philippinense and delenatii (which, based on pics of the hybrid Paph. Delphi made with alba parents, it appears to be) I would expect the ratio of alba to non-alba offspring to be 1:3. Also, the alba offspring would likely just be green and white, which many other breeders have already done.

Meanwhile the offspring of Aa Saint Swithin crossed to aa delenatii alba would have a ratio of about 1:1. Of course, I don’t know how robust the alba offspring are compared to normal color form, so I don’t imagine the survival rate would be equal. Also, parvi x multiflorals are tricky bloomers. But of the few that I get to bloom, there’s a shot at something special there— a flower free (or containing very little) anthocyanin, carotenoids, or chlorophyll. 🙂
 

Ernesto

ST Supporter
Supporting Member
Joined
Apr 21, 2020
Messages
158
Reaction score
79
Location
Manhattan, NY
What medium did you use?

When you replace make sure no ethanol touches the the plants or gets into the replates. It is better to use hydrogen peroxide as service sterilant while working with the flasks if you have limited space.
Half-strength P668 made with coconut water as a source of sugar. I followed Here But Not’s recipe for mother flasks.
 

Ernesto

ST Supporter
Supporting Member
Joined
Apr 21, 2020
Messages
158
Reaction score
79
Location
Manhattan, NY
Just an update on the mother flask— I’ve yet to replate, but many of the babies have begun forming leaves. Also worth noting that there are new protocorms germinating, and that some of them haven’t gotten the memo to start differentiating leaf tissue and have continued to grow in size.
 

Attachments

  • F034E21D-1F4C-41EB-A986-93C5D20C87F4.jpeg
    F034E21D-1F4C-41EB-A986-93C5D20C87F4.jpeg
    1.3 MB · Views: 0
  • 538E39C2-6BB3-4AB5-B856-E102D24E4460.jpeg
    538E39C2-6BB3-4AB5-B856-E102D24E4460.jpeg
    1.5 MB · Views: 0
  • 2A5FFCB6-CCA4-4D19-9733-79023D122C6F.jpeg
    2A5FFCB6-CCA4-4D19-9733-79023D122C6F.jpeg
    1.3 MB · Views: 1

Latest posts

Top