Advice needed kovachii seed sowing

Discussion in 'Breeding & Production' started by eteson, Jun 13, 2013.

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  1. Jun 13, 2013 #1

    eteson

    eteson

    eteson

    Phragmad

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    After years waiting.... I´ve got a "pod" of Pk :rollhappy:. I suspect that it is a selfing from an self-pollinated plant. The pod is impressive in size... almost 1.2 cm W x 7 cm L... and it is full of seed.
    I've read in this forum some advices from some time ago...
    I´ve read that Pk is hard to germinate, somewhere I read that it is really easy... somewhere that needs a medium with high pH... somewhere that the pH does not matter...
    I´ve been succesful with schlimii, besseae, lindenii, longifoluim and others using half strengh P668 + coconut water and very low agar concentration... but I am a bit scared about this sowing :confused: because I´ve been waiting for a long time to get the seed and I could take a long time to get more...

    Any advice is more than welcome
     
  2. Jun 14, 2013 #2

    eteson

    eteson

    eteson

    Phragmad

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    Pictures of the Phragmipedium kovachii seeds

    Almost 95% of the seed has viable embryos!

    Phragmipedium kovachii seed 40X @ Transmitted Light
    [​IMG]

    Phragmipedium kovachii seed 40X @ Reflected Light
    [​IMG]

    Phragmipedium kovachii seed 100X @ Transmitted Light
    [​IMG]

    Phragmipedium kovachii seed 100X @ Reflected Light
    [​IMG]
     
  3. Jun 14, 2013 #3

    eggshells

    eggshells

    eggshells

    Humongous Gnat

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    Hey that is very cool. I cant help but good luck to you.
     
  4. Jun 14, 2013 #4

    NYEric

    NYEric

    NYEric

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    Cool. IDK, maybe one of the breeders here will know.
     
  5. Jun 14, 2013 #5

    eteson

    eteson

    eteson

    Phragmad

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    In any case I am going to sow some of the seed tomorrow. I've prepared:
    P668 at half strenght (sugar corrected to 18g/l)
    100ml/l of coconut water
    3.5 gr/l of agar
    pH corrected to 6.0

    Sucess or Failure will be posted here.
     
  6. Jun 17, 2013 #6

    Trithor

    Trithor

    Trithor

    Chico (..... the clown)

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    Good luck! I look forward to a positive next post from you.
     
  7. Jun 17, 2013 #7

    eteson

    eteson

    eteson

    Phragmad

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    Thanks a lot Trithor.

    Two batches of seed were sow yesterday.
    The first as described above, the second one in liquid medium (as was adviced for Paphios by Xavier).

    Let´s wait a couple of weeks....
     
    Last edited: Jun 27, 2013
  8. Jun 17, 2013 #8

    The Orchid Boy

    The Orchid Boy

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    Anxious to hear the results!
     
  9. Jun 27, 2013 #9

    eteson

    eteson

    eteson

    Phragmad

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    Location:
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    First report:

    Testas are out, embryos growing in both media. :) (germination in dark conditions)

    In the first medium (P668 1/2 + CW) the embryos seem to be slightly more developped than in liquid media.

    No green color yet but everything seems to be going pretty well... fingers crossed.
     
    Last edited: Jun 28, 2013
  10. Jun 29, 2013 #10

    Trithor

    Trithor

    Trithor

    Chico (..... the clown)

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    Well done, it sounds as if you are having good success!
     
  11. Jun 29, 2013 #11

    Rick

    Rick

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    Wow that seems fast for germination.

    Very good!

    What was your sterilization procedure?
     
  12. Jun 30, 2013 #12

    eteson

    eteson

    eteson

    Phragmad

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    Hi Rick,

    The night before sowing I put the seed in a test tube containing water + table sugar, I put a rubber cap and apply vaccum with a syringe. (8h of vaccum treatment is enough)
    My standard sterilization procedure is 10 minutes in 10% bleach, 10 minutes in 5% bleach and one rinse in distilled water.
     
    Last edited: Jul 5, 2013
  13. Jul 19, 2013 #13

    eteson

    eteson

    eteson

    Phragmad

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    Update... some green!

    Sorry the poor quality of the pictures.

    [​IMG]

    [​IMG]

    The seed in liquid media is not going that fast.
     
  14. Jul 19, 2013 #14

    Trithor

    Trithor

    Trithor

    Chico (..... the clown)

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    That is great! Seeing this makes me impatient to get started myself! Congratulations.
     
  15. Jul 20, 2013 #15

    phrag guy

    phrag guy

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    they like to be cool
     
  16. Jul 22, 2013 #16

    eteson

    eteson

    eteson

    Phragmad

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    They are at 23°C (73F) 24h/day. This temperature works well for the germination and development of schlimii, besseae, andreettae... etc, and seems to me also fine for kovachii.
     
  17. Sep 4, 2013 #17
    That's fantastic! I would love to try and sow my own Phrags :) any chance of a new pic?
     
  18. Sep 6, 2013 #18

    eteson

    eteson

    eteson

    Phragmad

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    Hi Missgreen, If you do not have experience with in-vitro procedures I would advice to start with something easier... Phrags are not especially hard to germinate but need some "special cares"...

    This weekend I can take a new picture for you... they are going quite fine but much more slower than other phrags.
     
  19. Sep 8, 2013 #19
    Oh I'm not going to try and sow anything just yet :) next on the list is to get a flask of seedlings of some kind. But which orchids are easier than Phrags?
     
  20. Sep 8, 2013 #20
    ... to sow I mean :rollhappy:
     

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